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C321.ΔA.M9adapted
(Bacterial strain #98568)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 98568 Bacteria in agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    C321.deltaA genome after adaptive evolution in glucose minimal media (+ biotin)
  • Backbone size w/o insert (bp) 4600000
  • Modifications to backbone
    The full sequence of this evolved strain is included on NCBI (accession number CP025268)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    C321.deltaA
  • Growth instructions
    The C321.deltaA strain contains an integrated lambda red cassette under expression by heat shock. Growth at 34 C or lower is recommended to prevent leaky expression of lambda red.
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C321.ΔA.M9adapted was a gift from George Church (Addgene plasmid # 98568)
  • For your References section:

    Adaptive evolution of genomically recoded Escherichia coli. Wannier TM, Kunjapur AM, Rice DP, McDonald MJ, Desai MM, Church GM. Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3090-3095. doi: 10.1073/pnas.1715530115. Epub 2018 Feb 13. 10.1073/pnas.1715530115 PubMed 29440500