No maps are available for this item.
-
PurposeRecoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin
-
Depositing Lab
-
Sequence Information
-
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 98568 | Bacteria in agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backboneC321.deltaA genome after adaptive evolution in glucose minimal media (+ biotin)
- Backbone size w/o insert (bp) 4600000
-
Modifications to backboneThe full sequence of this evolved strain is included on NCBI (accession number CP025268)
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)C321.deltaA
-
Growth instructionsThe C321.deltaA strain contains an integrated lambda red cassette under expression by heat shock. Growth at 34 C or lower is recommended to prevent leaky expression of lambda red.
-
Copy numberUnknown
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
C321.ΔA.M9adapted was a gift from George Church (Addgene plasmid # 98568) -
For your References section:
Adaptive evolution of genomically recoded Escherichia coli. Wannier TM, Kunjapur AM, Rice DP, McDonald MJ, Desai MM, Church GM. Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3090-3095. doi: 10.1073/pnas.1715530115. Epub 2018 Feb 13. 10.1073/pnas.1715530115 PubMed 29440500