C321.Ub-UAG-sfGFP
(Bacterial strain
#98564)
No maps are available for this item.
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PurposeRecoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 98564 | Bacteria in agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneC321.deltaA genome (genomically integrated construct)
- Backbone size w/o insert (bp) 4600000
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Modifications to backboneA reporter construct was integrated into an intergenic region of the E. coli genome.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)C321.deltaA
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Growth instructionsThe C321.deltaA strain contains an integrated lambda red cassette under expression by heat shock. Growth at 34 C or lower is recommended to prevent leaky expression of lambda red.
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C321.Ub-UAG-sfGFP was a gift from George Church (Addgene plasmid # 98564) -
For your References section:
Engineering posttranslational proofreading to discriminate nonstandard amino acids. Kunjapur AM, Stork DA, Kuru E, Vargas-Rodriguez O, Landon M, Soll D, Church GM. Proc Natl Acad Sci U S A. 2018 Jan 16;115(3):619-624. doi: 10.1073/pnas.1715137115. Epub 2018 Jan 4. 10.1073/pnas.1715137115 PubMed 29301968