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PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFUGW
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Backbone manufacturerDavid Baltimore
- Total vector size (bp) 11498
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHistone 2B - green fluorescent protein fusion
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Alt nameH2B-GFP
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SpeciesSynthetic
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Insert Size (bp)714
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Tag
/ Fusion Protein
- GFP fusion (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene #26790; Fred Dick
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FU-H2B-GFP-IRES-Puro was a gift from Charles Gersbach (Addgene plasmid # 69550 ; http://n2t.net/addgene:69550 ; RRID:Addgene_69550)
Map uploaded by the depositor.
