166,481 results

1. pZac2.1-GfaABC1D-lck-jGCaMP8s (AAV1)

   Viral Prep

   #176761-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8s (#176761). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8s plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.n into animals.

   Depositor

   Loren Looger

   Promoter

   GfaABC1D

   Tags

   None

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

2. pDawn-sfGFP

   Plasmid

   #107741

   Purpose

   Expresses superfolder GFP under the control of a blue-light transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLambda

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7-VEE-mCherry

   Plasmid

   #193355

   Purpose

   Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-5xGCN4-P2A-BFP

   Plasmid

   #184438

   Purpose

   dCas9 fused with 5 copies of GCN4 epitope

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCR4-Shh::lacZ-H11

   Plasmid

   #139098

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

   Depositor

   Len Pennacchio

   Article

   Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Shh

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-BG4-NLS-mVen

   Plasmid

   #211449

   Purpose

   mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

   Depositor

   Helmut Pospiech

   Insert

   Anti-DNA G-quadruplex single chain antibody BG4
   

   Use

   Tags

   NLS-mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS_cAMPFIRE-M

   Plasmid

   #182284

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-M (cAMP sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-6xHis-MBP-TEV site-TadA8r

   Plasmid

   #208318

   Purpose

   Overxpression MBP-TadA8r

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2T-CMV-miniSdd7-BE4max-BlastR

   Plasmid

   #204851

   Purpose

   For cytosine base editing using miniSdd7 in mammalian cells

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd7-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV, SV40, EM7

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIEG3-JunDN

    Plasmid

    #40350

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

    Insert

    Jun dominant negative (JUN Human)
    

    Use

    Retroviral

    Tags

    HA and IRES eGFP

    Expression

    Mammalian

    Mutation

    first 122 amino acids deleted

    Promoter

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-MET-V5/HIS

    Plasmid

    #201982

    Purpose

    expression of human MET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MET receptor tyrosine kinase (MET Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe neo TGFBR3-HA

    Plasmid

    #83095

    Purpose

    Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    TGFBR3 (TGFBR3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

13. VE-cad-eGFP Donor plasmid

    Plasmid

    #92309

    Purpose

    Knock eGFP into human VE-cad locus

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad Homologous Recombination Arms (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    about 30 a.a. truncated at end of protein

    Promoter

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL0_5 [pAL5000 ORI]

    Plasmid

    #198922

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Cultivarium Tools, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pAL5000 ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. FH1-MGT#1-mVenus

    Plasmid

    #164094

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#1-mVenus
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHCD1B

    Plasmid

    #102953

    Purpose

    mammalian expression of CD1B

    Depositor

    Lucia Mori, Gennaro De Libero

    Article

    Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

    Insert

    CD1B cDNA (CD1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28.His.3C.Tau2N4R

    Plasmid

    #226396

    Purpose

    Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT Human, synthetic)
    

    Use

    Tags

    His.3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.2_mNeonGreen2

    Plasmid

    #113727

    Purpose

    Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Article

    Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

    Insert

    mNeonGreen2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJExpress414_GmPOPB

    Plasmid

    #92234

    Purpose

    Express wild type prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata
    

    Use

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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