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pMarC9-R6k
(Plasmid #89477)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89477 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAM1
  • Backbone manufacturer
    Victor de Lorenzo lab
  • Modifications to backbone
    Tn5 replaced with marC9. Mariner transposon ends cloned into the transposon cassette. The transposon ends are mutagenized to introduce an mmeI cut site.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    Use BW29427 strain supplemented with 300uM DAP for this plasmid when conjugating from E. coli to Vibrio natriegens
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pMarC9-R6k

Resource Information

Depositor Comments

BW29427 is an E. coli strain with diaminopimelic acid (DAP) auxotrophy. BW29427 growth requires 300uM DAP even when cultured in LB. Importantly, BW29427 does not grow in the absence of DAP, which simplifies counterselection of this host strain following biparental mating with Vibrio natriegens.

Please visit https://www.biorxiv.org/content/early/2016/06/12/058487 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMarC9-R6k was a gift from George Church (Addgene plasmid # 89477 ; http://n2t.net/addgene:89477 ; RRID:Addgene_89477)
  • For your References section:

    Vibrio natriegens, a new genomic powerhouse. Lee HH, Ostrov N, Wong BG, Gold MA, Khalil A, Church GM. bioRxiv 058487 10.1101/058487