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(Plasmid #89477)


Item Catalog # Description Quantity Price (USD)
Plasmid 89477 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Victor de Lorenzo lab
  • Modifications to backbone
    Tn5 replaced with marC9. Mariner transposon ends cloned into the transposon cassette. The transposon ends are mutagenized to introduce an mmeI cut site.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use BW29427 strain supplemented with 300uM DAP for this plasmid when conjugating from E. coli to Vibrio natriegens
  • Copy number


  • Gene/Insert name

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BW29427 is an E. coli strain with diaminopimelic acid (DAP) auxotrophy. BW29427 growth requires 300uM DAP even when cultured in LB. Importantly, BW29427 does not grow in the absence of DAP, which simplifies counterselection of this host strain following biparental mating with Vibrio natriegens.

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMarC9-R6k was a gift from George Church (Addgene plasmid # 89477 ; ; RRID:Addgene_89477)
  • For your References section:

    Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi. Lee HH, Ostrov N, Wong BG, Gold MA, Khalil AS, Church GM. Nat Microbiol. 2019 Apr 8. pii: 10.1038/s41564-019-0423-8. doi: 10.1038/s41564-019-0423-8. 10.1038/s41564-019-0423-8 PubMed 30962569