pET21b-Caspase-3
(Plasmid #90087)

• Purpose: Expresses wild type caspase 3 in bacterial cells
• Depositing Lab: Clay Clark
• Publication: Pop et al Biochemistry. 2001 Nov 27;40(47):14224-35.

Backbone

• Vector backbone: pET21b
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Caspase 3
• Species: H. sapiens (human)
• Mutation: Wild type
• Entrez Gene: CASP3 (a.k.a. CPP32, CPP32B, SCA-1)
• Tag / Fusion Protein:
  • His tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry