166,485 results

1. pMRS-LacI-GFP

   Plasmid

   #220198

   Purpose

   mini-Tn7 GFP expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   monomeric superfolder green fluorescent protein
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   LacI/pLac

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. OKSIM

   Plasmid

   #24603

   Depositor

   Jose Cibelli

   Article

   Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

   Insert

   Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Oct4-2A-KLF4-2A-Sox2-IRES-Myc

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Vav2

   Plasmid

   #129623

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   Vav2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

   Plasmid

   #176484

   Purpose

   TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-neo-HA-hUVRAG

   Plasmid

   #24290

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   UV irradiation resistance-associated gene (UVRAG Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 2, 2010

   Availability

   Academic Institutions and Nonprofits only

6. PB_iETV2_P2A_GFP_Puro

   Plasmid

   #168805

   Purpose

   PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   ETV2 (ETV2 Human, Synthetic)
   

   Use

   Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-CMV-Flag-TLK2_IDG-K

   Plasmid

   #135278

   Purpose

   Gateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-TLK2 (TLK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T-CMV-miniSdd6-BE4max-BlastR

   Plasmid

   #204850

   Purpose

   For cytosine base editing using miniSdd6 in mammalian cells

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd6-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV, SV40, EM7

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/Myc-DNMT3B1

   Plasmid

   #35522

   Purpose

   Mammalian expression of human DNMT3B1 with myc tag

   Depositor

   Arthur Riggs

   Article

   Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

   Insert

   DNMT3B1 (DNMT3B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. dSpCas9-VPR

    Plasmid

    #205247

    Purpose

    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-tdTomato (codon diversified) (AAV8)

    Viral Prep

    #59462-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    tdTomato (codon diversified)

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pMPRA2

    Plasmid

    #49350

    Purpose

    Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL5.0 shCAV1-NLS-mCherry

    Plasmid

    #187257

    Purpose

    Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Caveolin-1 (Cav1 Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-mCherry

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. FH1-MGT#2-mCherry

    Plasmid

    #164097

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#2-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. gTBEv5

    Plasmid

    #220621

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-Skp-TF

    Plasmid

    #83921

    Purpose

    Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    Trigger Factor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG2-EGFP-C-IFT144

    Plasmid

    #218740

    Purpose

    Expresses C-terminally EGFP-tagged IFT144 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT144 (WDR19 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcdna DSG-2 TS

    Plasmid

    #89465

    Purpose

    DSG-2 tension sensor, using TSmod inserted in human DSG-2

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. [UBC][NRE Pum][eGFP]

    Plasmid

    #97192

    Purpose

    The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    wild type Pumilio homology domain
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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