266,499 results

1. EIF2AK1 gRNA (BRDN0001162524)

   Plasmid

   #77050

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK1 (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. EIF2AK1 gRNA (BRDN0001162524)

   Plasmid

   #77050

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK1 (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-EPHA3

   Plasmid

   #116733

   Purpose

   Lentiviral expression of EPHA3

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EPHA3 (EPHA3 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-EPHA3

   Plasmid

   #116733

   Purpose

   Lentiviral expression of EPHA3

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EPHA3 (EPHA3 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmr101A-cPr-mCherry (ErmR)

   Plasmid

   #177206

   Purpose

   Plasmid with p15A origin for constitutive mCherry expression in E. coli

   Depositor

   Alexey Merz

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   Synthetic constitutive promoter

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF-hSEMA4C-Fc

   Plasmid

   #74449

   Purpose

   Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

   Depositor

   Roland Friedel

   Article

   Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

   Insert

   human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)
   

   Tags

   human Fc tag

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-hSEMA4C-Fc

   Plasmid

   #74449

   Purpose

   Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

   Depositor

   Roland Friedel

   Article

   Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

   Insert

   human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)
   

   Tags

   human Fc tag

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Tags

    VSV

    Expression

    Mammalian

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Tags

    VSV

    Expression

    Mammalian

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEasyG3_nat

    Plasmid

    #184918

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEasyG3_mic

    Plasmid

    #184920

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-mCherry-KATNA1

    Plasmid

    #180650

    Purpose

    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GGACAGCACUCCCUUGAAA

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ttr:mRFP1

    Plasmid

    #32607

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Kwon et al Genesis. 2009 Jul;47(7):447-55.

    Insert

    TTR:mRFP
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

16. Ttr:mRFP1

    Plasmid

    #32607

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Kwon et al Genesis. 2009 Jul;47(7):447-55.

    Insert

    TTR:mRFP
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

    Plasmid

    #193717

    Purpose

    Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Synthetic, Human)
    

    Tags

    2xmNeongreen-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF1825

    Plasmid

    #143322

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARNT (ARNT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1825

    Plasmid

    #143322

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARNT (ARNT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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