167,318 results

1. pGH044_mCherry

   Plasmid

   #85412

   Purpose

   lentiviral expression vector for mCherry

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHPHS800

   Plasmid

   #228240

   Purpose

   Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

   Insert

   Cas9
   

   Tags

   Hygromycin phosphotransferase

   Expression

   Mammalian

   Promoter

   none

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27-Tornado-Pepper-RhoBAST

   Plasmid

   #196343

   Purpose

   Overexpression of circular Pepper-RhoBAST

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   Tornado-Pepper-RhoBAST
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP2048 - pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA

   Plasmid

   #220727

   Purpose

   AiE2543m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-CalfluxVTN

   Plasmid

   #83926

   Purpose

   Bioluminescent Reporter of Intracellular Calcium

   Depositor

   Carl Johnson

   Article

   Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

   Insert

   CalfluxVTN
   

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM-ABE

   Plasmid

   #196292

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding ABE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad33_ISDge10_reRNA

   Plasmid

   #205340

   Purpose

   Bacterial expression of ISDge10 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISDge10 TnpB
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. V. natriegens NC1

   Bacterial Strain

   #215355

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pDEST17-UbE2R2

    Plasmid

    #15806

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pISH-Msx1

    Plasmid

    #84313

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx1. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    Msx1 (Msx1 Mouse)
    

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid

    #183410

    Purpose

    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    KRAB-dCas9-ecDHFR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. evoFERNY-Cas9NG

    Plasmid

    #203329

    Purpose

    Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    evoFERNY-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.2/EV

    Plasmid

    #120833

    Purpose

    Control vector, 20nt random sequence between gateway recombination sites

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWM_12x601_20bpLinker

    Plasmid

    #157787

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_20bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-FLAG-Claspin

    Plasmid

    #12659

    Depositor

    Michele Pagano

    Article

    Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

    Insert

    Claspin (CLSPN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pET-Impact-T4gp43(exo-)

    Plasmid

    #228202

    Purpose

    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A

    Depositor

    Stephen Benkovic

    Insert

    43
    

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated aspartate 219 to alanine (D219A), elimina…

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABEpuro mTurquoise2 LC3B

    Plasmid

    #78518

    Purpose

    Fluorescent reporter for LC3B visualization

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    retroviral

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV1)

    Viral Prep

    #162381-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pAWP78-PVCpnf1-16

    Plasmid

    #198271

    Purpose

    Unmodified pPVC; contains WT PVC structural/accessory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf1-16
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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