Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein.
Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia KC, Contag CH, Shen K, Blau HM, Lin MZ
Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888. PubMed Article
Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia KC, Contag CH, Shen K, Blau HM, Lin MZ
Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51309 | pcDNA3-mNeptune2 | Expresses far-red fluorescent protein mNeptune2 in mammalian cells |
| 51310 | pcDNA3-mNeptune2.5 | Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells |
| 51311 | pcDNA3-mCardinal | Expresses far-red fluorescent protein mCardinal in mammalian cells |
| 52631 | pNCS-mCardinal2 | bacterial expression vector with far-red FP mCardinal2 |
| 54590 | mCardinal-N1 | Localization: N1 Cloning Vector, Excitation: 604, Emission: 659 |
| 54663 | mCardinal-Lifeact-7 | Localization: Actin, Excitation: 604, Emission: 659 |
| 54799 | mCardinal-C1 | Localization: C1 Cloning Vector, Excitation: 604, Emission: 659 |
| 54800 | mCardinal-pBAD | Localization: Protein Expression Vector , Excitation: 604, Emission: 659 |
| 56151 | mCardinal-Actin-C-18 | Localization: Actin, Excitation: 604, Emission: 659 |
| 56152 | mCardinal-Alpha-Actinin-19 | Localization: Focal Adhesions / Actin, Excitation: 604, Emission: 659 |
| 56153 | mCardinal-CAF1-C-10 | Localization: Chromatin Assembly Factor 1, Excitation: 604, Emission: 659 |
| 56154 | mCardinal-Calnexin-N-14 | Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659 |
| 56155 | mCardinal-CENPB-N-22 | Localization: Nucleus/Centromeres, Excitation: 604, Emission: 659 |
| 56156 | mCardinal-Cx26-7 | Localization: Gap Junctions, Excitation: 604, Emission: 659 |
| 56157 | mCardinal-Cx43-7 | Localization: Gap Junctions, Excitation: 604, Emission: 659 |
| 56158 | mCardinal-CytERM-N-17 | Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659 |
| 56159 | mCardinal-Farnesyl-5 | Localization: Plasma Membrane, Excitation: 604, Emission: 659 |
| 56160 | mCardinal-Fibrillarin-7 | Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659 |
| 56161 | mCardinal-H1-10 | Localization: Nucleus/Histones, Excitation: 604, Emission: 659 |
| 56162 | mCardinal-H2B-C-10 | Localization: Nucleus/Histones, Excitation: 604, Emission: 659 |
| 56163 | mCardinal-H3-23 | Localization: Nucleus/Histones, Excitation: 604, Emission: 659 |
| 56164 | mCardinal-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659 |
| 56165 | mCardinal-LaminB1-10 | Localization: Nuclear Envelope, Excitation: 604, Emission: 659 |
| 56166 | mCardinal-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 604, Emission: 659 |
| 56167 | mCardinal-MannII-N-10 | Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659 |
| 56168 | mCardinal-MCAK-C-7 | Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659 |
| 56169 | mCardinal-Myotilin-C-14 | Localization: Cytoskeleton, Excitation: 604, Emission: 659 |
| 56170 | mCardinal-NUP50-N-10 | Localization: Nuclear Pore, Excitation: 604, Emission: 659 |
| 56171 | mCardinal-Paxillin-22 | Localization: Focal Adhesions, Excitation: 604, Emission: 659 |
| 56172 | mCardinal-PDHA1-N-10 | Localization: Mitochondrial Matrix, Excitation: 604, Emission: 659 |
| 56173 | mCardinal-PMP-N-10 | Localization: Peroxisomes, Excitation: 604, Emission: 659 |
| 56174 | mCardinal-Rab4a-7 | Localization: Ras GTPase, Excitation: 604, Emission: 659 |
| 56175 | mCardinal-Tensin1-N-18 | Localization: Focal Adhesions, Excitation: 604, Emission: 659 |
| 56176 | mCardinal-Tubulin-C-18 | Localization: Microtubules, Excitation: 604, Emission: 659 |
| 56178 | mCardinal-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 604, Emission: 659 |
| 56179 | mCardinal-ZO1-C-14 | Localization: Tight Junctions, Excitation: 604, Emission: 659 |