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(Plasmid #61041)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 61041 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pAd CMV/V5-DEST
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 36686
  • Total vector size (bp) 40200
  • Modifications to backbone
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Neurog3 (a.k.a. Atoh5, Math4B, bHLHa7, ngn3)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site salI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer ATGGCGCCTCATCCCT
  • 3′ sequencing primer GTTC TCAGACTTCTTG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Pdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer ATGAACAGTGAGGAG
  • 3′ sequencing primer CAGGAACCCCGG
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Mafa (a.k.a. RIPE3b1)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer ATGGCCGCGGAGC
  • 3′ sequencing primer CACCCGACTTCTTTCTG
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    monomeric cherry
  • Species
  • Insert Size (bp)

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer atggtgagcaagggcgagg
  • 3′ sequencing primer ggacgagctgtacaagtaa
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information, please see the associated article (Li, et al., Nat Biotechnol. 2014 PMID: 25402613.), as well as Li, et al., eLife. 2014, PMID: 24714494.

Note: The plasmid contains an N103Y amino acid residue substitution in the mouse Pdx1 gene. This residue change was present in the depositor's original plasmid stock as described in the associated publication and and should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-M3cherry was a gift from Qiao Zhou (Addgene plasmid # 61041 ; ; RRID:Addgene_61041)
  • For your References section:

    Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells. Li W, Cavelti-Weder C, Zhang Y, Clement K, Donovan S, Gonzalez G, Zhu J, Stemann M, Xu K, Hashimoto T, Yamada T, Nakanishi M, Zhang Y, Zeng S, Gifford D, Meissner A, Weir G, Zhou Q. Nat Biotechnol. 2014 Dec;32(12):1223-30. doi: 10.1038/nbt.3082. Epub 2014 Nov 17. 10.1038/nbt.3082 PubMed 25402613