This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pXPG
(Plasmid #71248)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71248 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pXP1 and pGL3
  • Backbone manufacturer
    Nordeen, 1988 and Promega
  • Modifications to backbone
    A single C to A mutation 564 bp upstream of the origin of replication at plasmid position 4660 was made to create pXPM. A HindIII/XbaI fragment of pGL3 (Promega) containing the improved luciferase gene Luc1 (Sherf et al.,1994) was cloned into into HindIII/XbaI-cut pXPM
  • Vector type
    Mammalian Expression, Luciferase
  • Promoter none
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

Depositor states "A spontaneous single C to A mutation was identified 564 bp upstream of the origin of replication at plasmid position 4660 in pXPM which generated high copy replication. A HindIII/XbaI fragment of pGL3 (Promega) containing the improved luciferase gene Luc+ (Sherf et al.,1994) was cloned into into HindIII/XbaI-cut pXPM."

JM109 are the cells used by the author for pXPG and its derivatives.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXPG was a gift from Peter Cockerill (Addgene plasmid # 71248)
  • For your References section:

    Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication. Bert AG, Burrows J, Osborne CS, Cockerill PN. Plasmid. 2000 Sep;44(2):173-82. S0147-619X(00)91474-4 [pii] PubMed 10964627