|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71248||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepXP1 and pGL3
Backbone manufacturerNordeen, 1988 and Promega
Modifications to backboneA single C to A mutation 564 bp upstream of the origin of replication at plasmid position 4660 was made to create pXPM. A HindIII/XbaI fragment of pGL3 (Promega) containing the improved luciferase gene Luc1 (Sherf et al.,1994) was cloned into into HindIII/XbaI-cut pXPM
Vector typeMammalian Expression, Luciferase
- Promoter none
/ Fusion Protein
- luciferase (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Depositor states "A spontaneous single C to A mutation was identified 564 bp upstream of the origin of replication at plasmid position 4660 in pXPM which generated high copy replication. A HindIII/XbaI fragment of pGL3 (Promega) containing the improved luciferase gene Luc+ (Sherf et al.,1994) was cloned into into HindIII/XbaI-cut pXPM."
JM109 are the cells used by the author for pXPG and its derivatives.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pXPG was a gift from Peter Cockerill (Addgene plasmid # 71248 ; http://n2t.net/addgene:71248 ; RRID:Addgene_71248)
For your References section:Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication. Bert AG, Burrows J, Osborne CS, Cockerill PN. Plasmid. 2000 Sep;44(2):173-82. S0147-619X(00)91474-4 [pii] PubMed 10964627