163,044 results

1. pZH82 pET28a-Rai1

   Plasmid

   #231651

   Purpose

   Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   rai1 (RAI1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZH23 Dual G-less containing ySSA1 polyA

   Plasmid

   #231647

   Purpose

   yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   SSA1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZH20 Dual G-less containing yCYC1 polyA

   Plasmid

   #231646

   Purpose

   yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   CYC1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB-3xFlag-dCas13-mTET2HxDCD

   Plasmid

   #228235

   Purpose

   For targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13

   Depositor

   Chuan He

   Article

   Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

   Inserts

   dCas13
   TET2 (Tet2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   CD (cysteine-rich, dioxygenase domain) truncation…

   Promoter

   CAG

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDM042

   Plasmid

   #216812

   Purpose

   Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mScarlet3
   

   Use

   Tags

   VinnyLinker

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-CAG-PlatTET-wpA-pPuroTK

   Plasmid

   #214125

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   dCas9-TET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC150-FAM134C-GFP

   Plasmid

   #201932

   Purpose

   PiggyBac Vector expressing FAM134C-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   FAM134C (RETREG3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP hSmad4-HomDonor

   Plasmid

   #206044

   Purpose

   Donor plasmid for CRISPR knock-in

   Depositor

   Ali Hemmati Brivanlou

   Article

   Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

   Insert

   Smad4 (SMAD4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193315

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Use

   Tags

   4FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMIMY

    Plasmid

    #191954

    Purpose

    A pMSCV-based backbone where the MCS has been substituted with a MCS-IRES-mitoYFP for expression of a gene of interest and a mitochondrially targeted YFP

    Depositor

    Luca Scorrano

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD/HisB (TIREVOL)-mNG

    Plasmid

    #189722

    Purpose

    titratable expression of mNeonGreen in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mNeonGreen
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL5.0 shFmnl2-EGFP

    Plasmid

    #187255

    Purpose

    Lentiviral expression of shRNA targeting Fmnl2

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Fmnl2 (Fmnl2 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJW1816

    Plasmid

    #154320

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KA0718_pPB-TREtight-cHA-IRESVenus

    Plasmid

    #124171

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RRM2 E4.3 gRNA

    Plasmid

    #90883

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM2 (Guide Designation E4.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. RRM2 E3.3 gRNA

    Plasmid

    #90882

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM2 (Guide Designation E3.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLPCX-VEcadTL

    Plasmid

    #45849

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    VE-cadherin (Cdh5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pM2s2TsR

    Plasmid

    #137923

    Purpose

    E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLOX-CWBmi1

    Plasmid

    #12240

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter upstream of mBmi-1. LoxP in 3' …

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-mCherry-CaM

    Plasmid

    #127393

    Purpose

    Fluorescent reporter for calmodulin 1

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    Calmodulin 1 (Calm1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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