167,318 results

1. pQE30-HyPer7

   Plasmid

   #141071

   Purpose

   Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Hisx6

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAC-LYC

   Plasmid

   #53270

   Purpose

   Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

   Insert

   crtE, crtI, crtB
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ALIX-mNeonGreen

   Plasmid

   #191191

   Purpose

   Full-length ALIX with C-terminal mNeonGreen tag.

   Depositor

   Jin Zhang

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX (PDCD6IP Human, Synthetic)
   

   Tags

   6xHIS and mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pArcCreER

   Plasmid

   #46389

   Depositor

   Liqun Luo

   Article

   Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

   Inserts

   Arc (Arc Mouse)
   CreERT2
   

   Use

   Mouse Targeting

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pH2B-emiRFP670

   Plasmid

   #136571

   Purpose

   Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   H2B-emiRFP670
   

   Tags

   emiRFP670

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSFDUET-1_VNp-anti-6xHis mAb

   Plasmid

   #214952

   Purpose

   Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-6xHis mAb heavy chain
   VNp-anti-6xHis mAb light chain
   

   Tags

   VNp6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. moxBFP

   Plasmid

   #68064

   Purpose

   C-terminal fusion to oxBFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Type

   Empty backbone

   Tags

   moxBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. E.coli_GW-sgRNA_Lib5

   Pooled Library

   #113138

   Purpose

   Genome-wide gRNA library for E. coli, sub5

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Expression

   Bacterial

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-FIP3

   Plasmid

   #74753

   Purpose

   Expressed sfGFP-tagged FIP3 in E. coli for protein production

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab11 family interacting protein 3 (RAB11FIP3 Human)
   

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. M-SPOTIT1.1

    Plasmid

    #171216

    Purpose

    Expresses a mu-opioid receptor-based circular permuted green fluorescent protein sensor

    Depositor

    Wenjing Wang

    Article

    Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

    Insert

    M-SPOTIT1.1
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSET-4xTR-TUBE

    Plasmid

    #110312

    Purpose

    E. coli expression and purification of 4xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    4xTR-TUBE (UBQLN1 Human)
    

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-CD63-L7Ae

    Plasmid

    #180503

    Purpose

    Human CD63-L7Ae fusion protein

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Human CD63-L7Ae fusion protein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB533-44B12m23-sfGFP

    Plasmid

    #186778

    Purpose

    RNAP2 Ser5ph-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    44B12m23-scFv
    

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSec61β-mScarlet3-H (aka mYongHong)

    Plasmid

    #239414

    Purpose

    Expresses Sec61b-mScarlet3-H in mammalian cells

    Depositor

    Zhifei Fu

    Article

    Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

    Insert

    Sec61β (SEC61B Human)
    

    Tags

    mScarlet3-H (aka mYongHong)

    Expression

    Mammalian

    Mutation

    M163H in mScarlet3

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid

    #181975

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p7SL-neo-TET

    Plasmid

    #51285

    Purpose

    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    7SL RNA (RN7SL2 Human)
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    7SL upstream pollII enhancer

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK2-AraE

    Plasmid

    #110141

    Purpose

    Constitutive promoter expressing araE on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    araE
    

    Expression

    Bacterial

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge