172,032 results

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PHB2-mCherry

Plasmid

#232785

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FlpO (AAV1)

Viral Prep

#183412-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-CAG-FlpO (#183412). In addition to the viral particles, you will also receive purified pAAV-CAG-FlpO plasmid DNA. CAG-driven expression of the recombinase FlpO. These AAV preparations are suitable purity for injection into animals.

Depositor

Janelia Viral Tools

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only
ancBE4-SpRY

Plasmid

#226855

Purpose

Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ancBE4max-SpRY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-sfGFP 150TAG

Plasmid

#197570

Purpose

Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
Pyl-tRNA (4 copies)

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sRGECO (AAV8)

Viral Prep

#137125-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-Ef1a-sRGECO (#137125). In addition to the viral particles, you will also receive purified pAAV-Ef1a-sRGECO plasmid DNA. EF1a-driven expression of cytosolic sRGECO calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

EF1a

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only
pJL96

Plasmid

#243684

Purpose

Stably expresses cytosolic LOV-BirA

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

LOV-BirA-GFP

Use

Lentiviral

Tags

3xFLAG

Promoter

EF1a

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pMK419 (GAL-OsTIR1(F74G))

Plasmid

#140656

Purpose

GAL-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74G)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS

Plasmid

#86677

Purpose

Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

EGFP-NLS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR9-YFP

Plasmid

#13642

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 9 (TLR9 Human)

Tags

YFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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sFlt1-e15a V5

Plasmid

#86045

Purpose

human Soluble Flt1 isoform e15a (terminates in exon 15a)

Depositor

Christie Thomas

Article

Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

Insert

human soluble Flt1 isoform e15a (FLT1 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

isoform e15a (terminates in exon 15a)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->sGFP-P2A-BFP

Plasmid

#162234

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 1 tet pLKO puro

Plasmid

#162985

Purpose

Tet-inducible shRNA targeting human METTL3 #1

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-p21-HA

Plasmid

#215110

Purpose

Expresses p21-HA in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV (Tet-inducible)

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

Plasmid

#85798

Purpose

donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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LV-AR

Plasmid

#213819

Purpose

Lenti-viral expression of human androgen receptor wild-type (WT).

Depositor

Steven Balk

Article

Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

Insert

AR (human androgen receptor) (AR Human)

Use

Lentiviral

Tags

Flag (N terminal on backbone)

Expression

Mammalian

Mutation

No, it is AR wild-type.

Promoter

EF-1a

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Long P2A

Plasmid

#164115

Purpose

Detect the long Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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