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Generating permissive site-specific unnatural aminoacyl-tRNA synthetases.
Miyake-Stoner SJ, Refakis CA, Hammill JT, Lusic H, Hazen JL, Deiters A, Mehl RA
Biochemistry. 2010 Mar 2;49(8):1667-77. doi: 10.1021/bi901947r.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
85482pBad-sfGFPArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag
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85483pBad-sfGFP 150TAGArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150
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85484pDule-tfmF A65V S158AMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
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Antibodies from Article