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MSCV-Luciferase-IRES-NrasG12D
(Plasmid #60834)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60834 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clonetech
  • Modifications to backbone
    All retroviruses were constructed in the MSCV backbone (Clontech). MSCV-IRES-Luciferase was generated by replacing GFP in MSCV-IRES-GFP with an NcoI/SalI fragment from pGL3-Basic (Promega) after excision of the 3′polyA signal. Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES.
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NrasG12D
  • gRNA/shRNA sequence
    NrasG12D
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nras (a.k.a. AV095280, N-ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco (unknown if destroyed)
  • 3′ cloning site Xho (unknown if destroyed)
  • 5′ sequencing primer MSCV
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-Luciferase-IRES-NrasG12D was a gift from Scott Lowe (Addgene plasmid # 60834 ; http://n2t.net/addgene:60834 ; RRID:Addgene_60834)
  • For your References section:

    Mouse models of human AML accurately predict chemotherapy response. Zuber J, Radtke I, Pardee TS, Zhao Z, Rappaport AR, Luo W, McCurrach ME, Yang MM, Dolan ME, Kogan SC, Downing JR, Lowe SW. Genes Dev. 2009 Apr 1;23(7):877-89. doi: 10.1101/gad.1771409. 10.1101/gad.1771409 PubMed 19339691