161,697 results

1. pMpGWB200

   Plasmid

   #68591

   Purpose

   MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGTag-eGFP-SV40

   Plasmid

   #117813

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB1532B-RFP

   Plasmid

   #101854

   Purpose

   Golden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

   Depositor

   Sophien Kamoun

   Article

   Pennington et al Figshare (2017)

   Type

   Empty backbone

   Use

   Fungal expression plasmid, tested in magnaporthe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEX-tTA

   Plasmid

   #149363

   Purpose

   AAV-mediated and Cre-dependent expression of tTA under the CAG promoter.

   Depositor

   Takeshi Imai

   Article

   Leiwe et al bioRxiv 2022.10.20.512984

   Insert

   tTA2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-HP1gamma

   Plasmid

   #17650

   Depositor

   Tom Misteli

   Article

   Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

   Insert

   HP1 gamma (CBX3 Human)
   

   Use

   Tags

   GFP and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. TVT7R(0,0)

   Plasmid

   #98631

   Purpose

   Vector for cloning infectious RNA virus genomes

   Depositor

   Karyn Johnson, Andrew Ball

   Article

   Johnson et al J Virol. 2000 Jun;74(11):5123-32.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK

   Plasmid

   #79120

   Purpose

   Lentiviral vector for constitutive expression of genes (PGK promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSFFV_mNG3K(1-10)

   Plasmid

   #157993

   Purpose

   Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

   Depositor

   Bo Huang

   Article

   Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

   Insert

   pSFFV_mNG3K(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G27D, D42E, M128I, K153M, T170I

   Promoter

   SFFV

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor14-ShHELIX-KanR (CJT70)

   Plasmid

   #181793

   Purpose

   pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)

    Plasmid

    #231733

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CTR9 (CTR9 Human)
    CDC73 (CDC73 Human)
    PAF1 (PAF1 Human)
    LEO1 (LEO1 Human)
    WDR61 (SKIC8 Human)
    

    Use

    Tags

    3C-Strep(II) and Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-DD-T6B-MUT-YFP

    Plasmid

    #235144

    Purpose

    Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    DHFR-fused mutant T6B peptide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    5 tryptophan residues of T6B-WT mutated

    Promoter

    hSyn

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAIP4_3KT9

    Plasmid

    #234192

    Purpose

    Heterologous protein expression of aprataxin in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3KT9 (APTX Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. AiP2107 - pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA

    Plasmid

    #230553

    Purpose

    AiE2664m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CA-FLEX-dtA

    Plasmid

    #232236

    Purpose

    Expresses diphtheria toxin A subunit in Cre-positive cells.

    Depositor

    Naoshige Uchida

    Article

    Tsutsui-Kimura et al bioRxiv 2022.02.05.479267

    Insert

    Diphtheria toxin A subunit
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CA

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a-Flex-MCS-WPRE-SV40 - GCH14

    Plasmid

    #230821

    Purpose

    AAV cloning vector for CRE dependent expression under the EF1a promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDL1403

    Plasmid

    #226635

    Purpose

    This plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.

    Depositor

    Andrew Camilli

    Article

    Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

    Inserts

    tfox
    qstR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

    Plasmid

    #212705

    Purpose

    targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system

    Depositor

    Elzo de Wit

    Insert

    ZFP143-FKBPV-2HA-P2A-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-memRFP-3xPax7gRNA

    Plasmid

    #224569

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid

    #224583

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTSAR3.1t

    Plasmid

    #210254

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mCerulean
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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