168,579 results

1. pFNMB1-msfGFP

   Plasmid

   #113191

   Purpose

   Mobilizable plasmid for tetracycline inducible gene expression in Francisella novicida

   Depositor

   Marek Basler

   Article

   Brodmann et al Front Cell Infect Microbiol. 2018 Aug 31

   Insert

   msfGFP
   

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET19-M(BRCT)TdTwt

   Plasmid

   #207459

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   M(BRCT)TdTwt (Dntt Mouse)
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTi4.0_SpdCas9_pmcDA1

   Plasmid

   #204613

   Purpose

   CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-SLC25A3_STOP

   Plasmid

   #161206

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A3 (SLC25A3 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLS13-IL-10

   Plasmid

   #209128

   Purpose

   Transient mammalian expression of IL-10

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   IL-10 (IL10 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. DH10B-ALT

   Bacterial Strain

   #61151

   Purpose

   DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Bacterial Resistance

   None

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRC-CMV-Rev1b

   Plasmid

   #164443

   Purpose

   Encodes HIV-1 Rev accessory protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   HIV-1 Rev
   

   Expression

   Mammalian

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB80-hsKIF5B(1-560)Rigor-L-mVenus

   Plasmid

   #193720

   Purpose

   Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Human, Synthetic)
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEmerald-CLIP170-N-18

    Plasmid

    #54044

    Purpose

    Localization: Microtubule Binding , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CLIP170 (CLIP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pJG/BETA MHC promoter

    Plasmid

    #53963

    Purpose

    transgene expression driven by BETA MHC promoter

    Depositor

    Jeffrey Robbins

    Article

    Rindt et al J Biol Chem. 1993 Mar 5;268(7):5332-8.

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    beta MHC

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+) eGFP PPP1R8 G4

    Plasmid

    #129021

    Purpose

    Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) eGFP EML2 G4

    Plasmid

    #129023

    Purpose

    Expresses an eGFP mRNA with the EML2 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEP4 E02S EM2K

    Plasmid

    #20923

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
    

    Use

    Episomal/ebna

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGCGFP-puro

    Plasmid

    #31266

    Depositor

    Andrew Pierce

    Insert

    GCGFP
    

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167849

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

    Plasmid

    #170712

    Purpose

    dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Inserts

    mNeonGreen
    mScarlet
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TNNT2 and aMHC

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LbCas12a-niCPE

    Plasmid

    #213747

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-nLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A in LbCas12a

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGD_RT_SRDX

    Plasmid

    #136976

    Purpose

    Goldengate/Greengate module EAR-repression domain

    Depositor

    Klaus Theres

    Insert

    SRDX
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF2703

    Plasmid

    #144151

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ESRRA (ESRRA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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