168,579 results

1. pCAG-MBP-ATG9 (692-839)

   Plasmid

   #188092

   Purpose

   To make monomeric ATG9 C-terminal tail for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (692-839)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_YES1_SH3

   Plasmid

   #178492

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   YES1_SH3 (YES1 Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmr101A-cPr-mNeonGreen (ErmR)

   Plasmid

   #177208

   Purpose

   Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

   Depositor

   Alexey Merz

   Insert

   mNeonGreen
   

   Expression

   Bacterial

   Mutation

   Yeast codon optimized

   Promoter

   Synthetic constitutive promoter

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY153: Flag(SUZ12) WT HDR plasmid

   Plasmid

   #170793

   Purpose

   WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   SUZ12 (SUZ12 Human)
   

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

   Plasmid

   #170792

   Purpose

   This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) eGFP SH3BP5 G4

   Plasmid

   #129022

   Purpose

   Expresses an eGFP mRNA with the SH3BP5 G-quadruplex (G4) region in the 3' UTR

   Depositor

   Jeremy Wilusz

   Article

   Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

   Insert

   eGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. BFP-Dcp1a

   Plasmid

   #153973

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1a

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1a (DCP1A Human)
   

   Tags

   mTagBFP-c1

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET GFP LIC cloning vector (2GFP-T)

   Plasmid

   #29716

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, SiriusGFP, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. ER-mit RspA-splitFAST (lentiviral plasmid)

   Plasmid

   #233604

   Purpose

   Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-splitFAST
   

   Use

   Lentiviral

   Promoter

   CMV/SV40

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57-MCS1-P2A-MCS2-mDux

    Plasmid

    #138320

    Purpose

    Dox-inducible codon-optimized mouse Dux

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    Dux (Dux Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Beclin1

    Plasmid

    #21150

    Depositor

    Junying Yuan

    Article

    Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

    Insert

    PcDNA3-Beclin1 (BECN1 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-SaCas9-U6-sgGrin1

    Plasmid

    #124852

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAAV-anti-PDL1(KN035) PAGER(TF)

    Plasmid

    #230003

    Purpose

    Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR223-BALF4

    Plasmid

    #195835

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BALF4

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF4 (BALF4 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CamKII(1.3).eYFP.WPRE.hGH (AAV1)

    Viral Prep

    #105622-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.CamKII(1.3).eYFP.WPRE.hGH (#105622). In addition to the viral particles, you will also receive purified pAAV.CamKII(1.3).eYFP.WPRE.hGH plasmid DNA. Expression of EYFP from CaMKII(1.3) promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII(1.3)

    Tags

    EYFP

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

17. Emerin pEGFP-C1 (637)

    Plasmid

    #61993

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Emerin (EMD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-ccdB-boxB-tBE-V5-mA3

    Plasmid

    #171693

    Purpose

    Expresses tBE-V5-mA3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and EF1a

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR_Pax6-RE_tBFP_PGK_mCitrine

    Plasmid

    #188388

    Purpose

    Lentiviral vector - tBFP reporter for Pax6-based SNIPRs with a constitutive mCitrine

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    Pax6-RE_tBFP_PGK_mCitrine
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHS_Nova

    Plasmid

    #239831

    Purpose

    NovaIscB mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    NovaIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Insertion of REC domain from NbaCas9-1 and swap o…

    Promoter

    CMV

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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