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Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens.
Najm FJ, Strand C, Donovan KF, Hegde M, Sanson KR, Vaimberg EW, Sullender ME, Hartenian E, Kalani Z, Fusi N, Listgarten J, Younger ST, Bernstein BE, Root DE, Doench JG
Nat Biotechnol. 2018 Feb;36(2):179-189. doi: 10.1038/nbt.4048. Epub 2017 Dec 18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
96917pXPR_120for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR
96919pXPR_123for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.
96920pXPR_206for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold
96921pPapiexpression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

Antibodies from Article