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An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation.
Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP
FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
72928pNatA (pACYCduet-naa10-naa15)Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker
72929pETpRha-alpha-SkTmAllows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoter
87855pET-alpha-synuclein-BiFCpETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation
87856pET-BiFCpETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
99359pJC20-Tpm4.2 (Rn)Bacterial expression vector containing cDNA encoding for the Rattus norvegicus tropomyosin, Tpm4.2 under the control of the pRhamnose promoter
110061pDUET-1-alpha-synuclein-mCerulean3-His6For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

Antibodies from Article