pJC20-Tpm4.2 (Rn)
(Plasmid #99359)

• Purpose: Bacterial expression vector containing cDNA encoding for the Rattus norvegicus tropomyosin, Tpm4.2 under the control of the pRhamnose promoter
• Depositing Lab: Dan Mulvihill
• Publication: Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597.

Backbone

• Vector backbone: pET3a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4674
• Total vector size (bp): 5529
• Modifications to backbone: T7 promoter replaced with Rhamnose inducible promoter
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tpm4.2
• Species: R. norvegicus (rat)
• Insert Size (bp): 747
• Entrez Gene: Tpm4 (a.k.a. Tpm4.2, Tpm4.2cy)
• Promoter: Rhamnose

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry