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pNatA (pACYCduet-naa10-naa15)
(Plasmid #72928)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72928 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYCDUET
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4008
  • Total vector size (bp) 6376
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    naa10
  • Species
    S. pombe (fission yeast), Synthetic
  • Insert Size (bp)
    534
  • Mutation
    Codon optomised for E.coli expression
  • Entrez Gene
    naa10 (a.k.a. SPAC15E1.08, ard1)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer DuetUP2 Primer
  • 3′ sequencing primer T7 Terminator Primer
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    naa15
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    2190
  • Entrez Gene
    naa15 (a.k.a. SPCC338.07c)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sac1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1 Primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNatA (pACYCduet-naa10-naa15) was a gift from Dan Mulvihill (Addgene plasmid # 72928 ; http://n2t.net/addgene:72928 ; RRID:Addgene_72928)
  • For your References section:

    An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation. Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP. FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597. 10.1002/1873-3468.12597 PubMed 28214355