pDUET-1-alpha-synuclein-mCerulean3-His6
(Plasmid #110061)

• Purpose: For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines
• Depositing Lab: Dan Mulvihill
• Publication: Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597.

Backbone

• Vector backbone: pDUET-1
• Total vector size (bp): 2924
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha-synuclein
• Species: H. sapiens (human)
• Insert Size (bp): 1179
• Entrez Gene: SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
• Promoter: T7
• Tags / Fusion Proteins:
  • mCerulean3 (C terminal on insert)
  • 6 x His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry