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177,491 results

Showing: 7521 - 7540 of 177491 results

  2. PB-CMV-MCS-Puro

    Plasmid
    #219794
    Purpose
    Mammalian expression plasmids using piggyBac Transposon System
    Depositor
    Keiichi Nakayama
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219794-plasmid-map-sequence-id-441249

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  4. pGD-p19

    Plasmid
    #196326
    Purpose
    35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    tomato bushy stunt virus p19
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196326-plasmid-map-sequence-id-390023

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  8. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and TRE3G
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  10. pAAV-Ef1a-Con/Fon-GCaMP6F (AAV8)

    Viral Prep
    #137122-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-GCaMP6F (#137122). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-GCaMP6F plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available Since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only

  13. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  14. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV2)

    Viral Prep
    #100054-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CAG
    Tags
    mCherry
    Available Since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  15. pMH-Halo tag

    Plasmid
    #154144
    Purpose
    Gateway compatible mammalian expression plasmid for halo tag fusion proteins
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    Halo tag
    Expression
    Mammalian
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154144-plasmid-map-sequence-id-309445

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  16. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139836-plasmid-map-sequence-id-308228

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  17. pLenti-PalmGRET

    Plasmid
    #158221
    Purpose
    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.
    Depositor
    Charles P. Lai
    Article
    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014
    Insert
    PalmGFP-Nluc
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    158221-plasmid-map-sequence-id-309022

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  18. AAV SYN PSAM4 GlyR IRES EGFP (AAV5)

    Viral Prep
    #119742-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV SYN PSAM4 GlyR IRES EGFP (#119742). In addition to the viral particles, you will also receive purified AAV SYN PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Scott Sternson
    Article
    Magnus et al Science. 2019 Mar 14. pii: science.aav52
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (AAV1)

    Viral Prep
    #84445-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (#84445). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] plasmid DNA. CAG-driven, Cre-dependent Jaws-GFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    CAG
    Tags
    GFP (Cre-dependent)
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 7521 - 7540 of 177491 results