168,579 results

1. pSB90 - pAGM4723_VirGN54D

   Plasmid

   #123187

   Purpose

   Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Type

   Empty backbone

   Use

   Level 2 acceptor vector for expression in plants

   Expression

   Plant

   Mutation

   VirGN54D for increase virulence

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1)

   Viral Prep

   #52473-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-synP-FLEX-splitTVA-EGFP-B19G (#52473). In addition to the viral particles, you will also receive purified pAAV-synP-FLEX-splitTVA-EGFP-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ian Wickersham

   Article

   Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

   Promoter

   Syn

   Tags

   EGFP (Cre-dependent)

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

3. FUGW-d2GFP-ZEB1 3'UTR

   Plasmid

   #79601

   Purpose

   FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

   Depositor

   Jeffrey Rosen

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   d2GFP-Zeb1 3'UTR
   

   Use

   Lentiviral

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (AAV8)

   Viral Prep

   #137163-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (#137163). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-EYFP for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Cre-dependent)

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pBT120a17

   Plasmid

   #138510

   Purpose

   P1

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   gIII-xLuxAB
   

   Use

   Synthetic Biology

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Vimentin-7

   Plasmid

   #55156

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Vimentin (VIM Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

7. MSP573

   Plasmid

   #98796

   Purpose

   Expression Vector

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   pET28 MBP-ENLYFQS-TEV WT
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-GRAB_CRF1.0

   Plasmid

   #208661

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-His6-FnCas9GFP

   Plasmid

   #130966

   Purpose

   expression of FnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   HA-NLS-FnCas9
   

   Use

   CRISPR

   Tags

   6xHis-mEGFP-TEV-HA-NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330A_dCas9-1x4

    Plasmid

    #63598

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Flag Erg

    Plasmid

    #66977

    Purpose

    Expression plasmid for mouse Erg; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Erg (Erg Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-6xHis-GFP(-30)

    Plasmid

    #199167

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(-30)
    

    Tags

    MGHHHHHHGGA

    Mutation

    T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP VAMP8

    Plasmid

    #42311

    Depositor

    Thierry Galli

    Article

    Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

    Insert

    VAMP 8 (VAMP8 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC015 - dLwCas13a-NF

    Plasmid

    #91905

    Purpose

    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

    Expression

    Mammalian

    Mutation

    R474A and R1046A

    Promoter

    CAG

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_NEmut (AAV9)

    Viral Prep

    #123310-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NEmut (#123310). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NEmut plasmid DNA. hSyn-driven expression of GRAB-NEmut control norepinephrine sensor (does not respond to NE). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Promoter

    Syn

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035)

    Plasmid

    #214853

    Purpose

    Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs)

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    CoChR-EGFP
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF3427

    Plasmid

    #144903

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIB (NFIB Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDV044

    Plasmid

    #191824

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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