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Addgene

pSIE1
(Plasmid #136355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136355 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pExchange_tdk
  • Backbone size w/o insert (bp) 2980
  • Total vector size (bp) 6196
  • Vector type
    Unspecified
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Type VI effector bfe1
  • Species
    Bacteroides fragilis 638R
  • Insert Size (bp)
    993
  • Mutation
    ATG removed to add signal sequence before gene
  • Promoter P1TDP-GHO23

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Type VI effector bte1
  • Species
    Bacteroides fragilis NCTC9343
  • Insert Size (bp)
    864
  • Mutation
    ATG removed to add signal sequence before gene
  • Promoter P1TDP-GHO23

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    BT_4676
  • Alt name
    Periplasmic signal sequence
  • Species
    Bacteroides thetaiotaomicron VPI5482
  • Insert Size (bp)
    63

Gene/Insert 4

  • Gene/Insert name
    tetR
  • Species
    E. coli
  • Insert Size (bp)
    624

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIE1 was a gift from Andrew Goodman (Addgene plasmid # 136355 ; http://n2t.net/addgene:136355 ; RRID:Addgene_136355)
  • For your References section:

    Genetic Manipulation of Wild Human Gut Bacteroides. Bencivenga-Barry NA, Lim B, Herrera CM, Trent MS, Goodman AL. J Bacteriol. 2020 Jan 15;202(3). pii: JB.00544-19. doi: 10.1128/JB.00544-19. Print 2020 Jan 15. 10.1128/JB.00544-19 PubMed 31712278