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(Plasmid #126618)


Item Catalog # Description Quantity Price (USD)
Plasmid 126618 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    1) Chemically synthesized cassette (Genscript) containing a periplasmic localization signal (first 63 bp of BT_4676 from B. thetaiotaomicron VPI-5482) fused to the bfe1 gene (BF638R_1988) of B. fragilis 638R. (Lim et al., 2017) 2) Using NEBuilder assembly, this cassette was cloned into the NcoI site of pNBU2_erm-TetR-P1T_DP-GH023. 3) The resulting plasmid was used as template for PCR , to remove the attN2 site and the integrase gene, and the resulting product was assembled using NEBuilder.
  • Vector type
    allellic exchange in bacteria
  • Selectable markers
    erythromycin (Bacteroidales)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    S17-lambda pir
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLGB13 was a gift from Laurie Comstock (Addgene plasmid # 126618 ; ; RRID:Addgene_126618)
  • For your References section:

    Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota. Garcia-Bayona L, Comstock LE. mBio. 2019 Aug 13;10(4). pii: mBio.01762-19. doi: 10.1128/mBio.01762-19. 10.1128/mBio.01762-19 PubMed 31409684