-
Purpose(Empty Backbone) pNBU2-derived shuttle vector for integration at attBT2 sites in Bacteroides chromosomes, inulin selection for use in anbitiotic resistant strains
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepNBU2
-
Modifications to backboneNEBuilder assembly reaction to fuse: a) pNBU2 backbone removing ermG cassette b) PBT1311 promoter and RBS, amplified from pMM553 (Mimee et al., 2015)(this promoter will drive expression of the inulin cassette) c) BACOVA_04504 and BACOVA_04505 from B. ovatus ATCC 8483 d) BACOVA_04501 from B. ovatus ATCC 8483
-
Vector typeUnspecified ; chromosomal integration suicide vector
-
Selectable markersinulin (Bacteroidales)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)S17-lambda pir
-
Copy numberLow Copy
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLGB28 was a gift from Laurie Comstock (Addgene plasmid # 126617 ; http://n2t.net/addgene:126617 ; RRID:Addgene_126617) -
For your References section:
Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota. Garcia-Bayona L, Comstock LE. mBio. 2019 Aug 13;10(4). pii: mBio.01762-19. doi: 10.1128/mBio.01762-19. 10.1128/mBio.01762-19 PubMed 31409684
