pNBU2_erm_SIE1
(Plasmid #136356)

• Purpose: Selection by Inducible Effectors Test Plasmid
• Depositing Lab: Andrew Goodman
• Depositing Organization: Yale University
  Located in the United States of America
• Publication: Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB.00544-19. doi: 10.1128/JB.00544-19. Print 2020 Jan 15.
• Citations: 1 Article

Backbone

• Vector backbone: pNBU2
• Backbone size w/o insert (bp): 4909
• Total vector size (bp): 8127
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Type VI effector bfe1
• Species: Bacteroides fragilis 638R
• Insert Size (bp): 993
• Mutation: ATG removed to add signal sequence before gene

Gene/Insert 2

• Gene/Insert name: Type VI effector bte1
• Species: Bacteroides fragilis NCTC9343
• Insert Size (bp): 864
• Mutation: ATG removed to add signal sequence before gene

Gene/Insert 3

• Gene/Insert name: BT_4676
• Alt name: Periplasmic signal sequence
• Species: Bacteroides thetaiotaomicron VPI5482
• Insert Size (bp): 63

Gene/Insert 4

• Gene/Insert name: tetR
• Species: E. coli
• Insert Size (bp): 624

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 136356-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 136356-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.