Addgene

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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Red cGull

Plasmid

#191853

Purpose

Expresses biosensor for cGMP in mammalian cells

Depositor

Takashi Tsuboi

Article

Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

Insert

Red cGull

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Red cGull

Plasmid

#191853

Purpose

Expresses biosensor for cGMP in mammalian cells

Depositor

Takashi Tsuboi

Article

Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

Insert

Red cGull

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E-elavl3

Plasmid

#75025

Purpose

Can be used to drive pan neuronal expression in zebrafish.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)

Use

5' entry vector for multisite gateway three …

Tags

Expression

Mutation

Promoter

elavl3

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-elavl3

Plasmid

#75025

Purpose

Can be used to drive pan neuronal expression in zebrafish.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)

Use

5' entry vector for multisite gateway three …

Tags

Expression

Mutation

Promoter

elavl3

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT374

Plasmid

#125615

Purpose

Expresses evoFERNY-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

H102P D104N

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT374

Plasmid

#125615

Purpose

Expresses evoFERNY-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

H102P D104N

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX_311-KRAB-dCas9

Plasmid

#96918

Purpose

for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

Depositor

David Root, John Doench, William Hahn

Article

Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

Insert

dCas9

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX_311-KRAB-dCas9

Plasmid

#96918

Purpose

for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

Depositor

David Root, John Doench, William Hahn

Article

Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

Insert

dCas9

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PB533-42B3mutAC2-sfGFP

Plasmid

#186777

Purpose

RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

Insert

42B3mutAC2-scFv

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB533-42B3mutAC2-sfGFP

Plasmid

#186777

Purpose

RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

Insert

42B3mutAC2-scFv

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-DJ8R

Plasmid

#213974

Purpose

AAV packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid coding sequence

Depositor

Zhonghua Lu

Article

Chen et al Neurosci Bull. 2023 Jul 11. doi: 10.1007

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-DJ8R

Plasmid

#213974

Purpose

AAV packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid coding sequence

Depositor

Zhonghua Lu

Article

Chen et al Neurosci Bull. 2023 Jul 11. doi: 10.1007

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock2 (Rock2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock2 (Rock2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLifeAct-mScarlet3_N1

Plasmid

#189767

Purpose

Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LifeAct-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLifeAct-mScarlet3_N1

Plasmid

#189767

Purpose

Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LifeAct-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge