pcDNA3 Plk4(Sak) wt (Nigg HR9)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41165||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1/3x myc-A
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 8400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt namepolo-like kinase 4
SpeciesH. sapiens (human)
Insert Size (bp)2900
- Promoter CMV
/ Fusion Proteins
- 3xMyc (N terminal on backbone)
- PreScission Site (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Polymerase chain reaction was used to amplify full-length human PLK4 from the expressed sequence tag AI561146 clone IMAGE:2210962 (RZPD, Berlin, Germany). The cDNA was then subcloned into mammalian expression vector pcDNA3.1/3x myc-A providing an N-terminal Myc tag. Construct was verified by sequencing.
Addgene NGS results found additional amino acids - ESRGPV - at the very C-terminus of the insert compared to the NCBI reference [AAX43264.1], but construct should function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 Plk4(Sak) wt (Nigg HR9) was a gift from Erich Nigg (Addgene plasmid # 41165 ; http://n2t.net/addgene:41165 ; RRID:Addgene_41165)
For your References section:The Polo kinase Plk4 functions in centriole duplication. Habedanck R, Stierhof YD, Wilkinson CJ, Nigg EA. Nat Cell Biol. 2005 Nov;7(11):1140-6. 10.1038/ncb1320 PubMed 16244668