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pEGFP Rootletin (Nigg pFL2(CW499))
(Plasmid #41166)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41166 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCJW201 (modified pEGFP-C1)
  • Backbone manufacturer
    Modified from Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 10700
  • Modifications to backbone
    FseI- and NotI-site-containing linker (AAT TCG GCC GGC CTA GCG GCC GCT GCA) inserted between EcoRI and PstI sites.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rootletin
  • Alt name
    CROCC
  • Alt name
    ciliary rootlet coiled-coil, rootletin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6000
  • GenBank ID
  • Entrez Gene
    CROCC (a.k.a. ROLT, TAX1BP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EGFP-C; CMV-F
  • 3′ sequencing primer SV40-pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Partial cDNA clone KIAA0445 from Kazusa DNA Research Institute and IMAGE clone 6150861 from Deutsches Ressourcenzentrum fuer Genomforschung GmbH
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The partial cDNA clones KIAA0445 (Kazusa DNA Research Institute) and IMAGE clone 6150861 (Deutsches Ressourcenzentrum fuer Genomforschung GmbH) were fused, and the complete coding sequence for rootletin was cloned into pBluescript II SK- (Addgene plasmid #41168) and subsequently subcloned into pEGFP-C1 (CLONTECH Laboratories, Inc.). All constructs were confirmed by sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP Rootletin (Nigg pFL2(CW499)) was a gift from Erich Nigg (Addgene plasmid # 41166 ; http://n2t.net/addgene:41166 ; RRID:Addgene_41166)
  • For your References section:

    Rootletin forms centriole-associated filaments and functions in centrosome cohesion. Bahe S, Stierhof YD, Wilkinson CJ, Leiss F, Nigg EA. J Cell Biol. 2005 Oct 10;171(1):27-33. Epub 2005 Oct 3. 10.1083/jcb.200504107 PubMed 16203858