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pEGFP Cep164 (Nigg CW324)
(Plasmid #41149)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41149 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCJW204 (modified pEGFP-N3)
  • Backbone manufacturer
    Modified from Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 9100
  • Modifications to backbone
    FseI- and NotI-site-containing linker (AAT TCG GCC GGC CTA GCG GCC GCT GCA) inserted between EcoRI and PstI sites.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cep164
  • Alt name
    centrosomal protein 164kDa
  • Alt name
    NPHP15
  • Alt name
    KIAA1052
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4400
  • GenBank ID
    NM_014956.4 AB028975
  • Entrez Gene
    CEP164 (a.k.a. NPHP15)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N; SV40-pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human Cep164 cDNA from clone KIAA1052 (Kazusa DNA Research Institute).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR was used to amplify a full-length human Cep164 cDNA from clone KIAA1052 (Kazusa DNA Research Institute).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP Cep164 (Nigg CW324) was a gift from Erich Nigg (Addgene plasmid # 41149 ; http://n2t.net/addgene:41149 ; RRID:Addgene_41149)
  • For your References section:

    Cep164, a novel centriole appendage protein required for primary cilium formation. Graser S, Stierhof YD, Lavoie SB, Gassner OS, Lamla S, Le Clech M, Nigg EA. J Cell Biol. 2007 Oct 22;179(2):321-30. 10.1083/jcb.200707181 PubMed 17954613