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GABA(A) receptor subunit B3SE
(Plasmid #49171)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49171 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    modified pRK5
  • Backbone manufacturer
    BD PharMingen
  • Total vector size (bp) 7097
  • Modifications to backbone
    region 5’ of the cloning site is slightly different
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    GABA(A) receptor subunit beta 3
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    GTG (V) to TGC (C)
  • GenBank ID
  • Entrez Gene
    Gabrb3 (a.k.a. A230092K12Rik, Cp1, Gabrb-3, beta3)
  • Promoter CMV
  • Tags / Fusion Proteins
    • pHGFP (pHluorin) (N terminal on insert)
    • myc (N terminal on insert)
    • thrombin cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer P5
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Deletion of YK at end of pHGFP, added extra base to keep frame before myc-tag

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GABA(A) receptor subunit B3SE was a gift from Tija Jacob & Stephen Moss (Addgene plasmid # 49171 ; ; RRID:Addgene_49171)
  • For your References section:

    Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors. Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG, Moss SJ. J Neurosci. 2005 Nov 9;25(45):10469-78. 10.1523/JNEUROSCI.2267-05.2005 PubMed 16280585