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GABA(A) receptor subunit a2SE
(Plasmid #49169)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49169 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    modified pRK5
  • Backbone manufacturer
    BD PharMingen
  • Backbone size w/o insert (bp) 5084
  • Modifications to backbone
    region 5’ of the cloning site is slightly different
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    GABA(A) receptor subunit alpha-2
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
  • GenBank ID
  • Entrez Gene
    GABRA2 (a.k.a. DEE78, EIEE78)
  • Promoter CMV
  • Tags / Fusion Proteins
    • bovine alpha 1 signal sequence (N terminal on insert)
    • HA (N terminal on insert)
    • pHGFP (pHIuorin) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer P5
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GABA(A) receptor subunit a2SE was a gift from Tija Jacob & Stephen Moss (Addgene plasmid # 49169 ; ; RRID:Addgene_49169)
  • For your References section:

    The clustering of GABA(A) receptor subtypes at inhibitory synapses is facilitated via the direct binding of receptor alpha 2 subunits to gephyrin. Tretter V, Jacob TC, Mukherjee J, Fritschy JM, Pangalos MN, Moss SJ. J Neurosci. 2008 Feb 6;28(6):1356-65. doi: 10.1523/JNEUROSCI.5050-07.2008. 10.1523/JNEUROSCI.5050-07.2008 PubMed 18256255