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Addgene

GABA(A) receptor subunit g2SE
(Plasmid #49170)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49170 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    modified pRK5
  • Backbone manufacturer
    BD PharMingen
  • Total vector size (bp) 7557
  • Modifications to backbone
    region 5’ of the cloning site is slightly different
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GABA(A) receptor subunit gamma 2
  • Alt name
    Gabrg2
  • Species
    M. musculus (mouse)
  • GenBank ID
  • Entrez Gene
    Gabrg2 (a.k.a. GABAA-R, Gabrg-2, gamma2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (N terminal on insert)
    • pHGFP (pHluorin) (N terminal on insert)
    • thrombin cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer P5
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

myc and pHGFP are inserted into coding sequence of gamma2 subunit.
R to T mutation (AGG-ACG) does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GABA(A) receptor subunit g2SE was a gift from Tija Jacob & Stephen Moss (Addgene plasmid # 49170 ; http://n2t.net/addgene:49170 ; RRID:Addgene_49170)
  • For your References section:

    Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors. Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG, Moss SJ. J Neurosci. 2005 Nov 9;25(45):10469-78. 10.1523/JNEUROSCI.2267-05.2005 PubMed 16280585