pCMV rat SAPAP1
(Plasmid
#47940)
-
PurposeExpresses rat SAPAP1 in mammalian cells
-
Depositing Labs
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepCMV5
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSAPAP1
-
SpeciesR. norvegicus (rat)
-
Entrez GeneDlgap1 (a.k.a. DAP-1, GKAP/SAPAP1, Gkap, SAPAP1, rGKAP)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SmaI or XmaI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV rat SAPAP1 was a gift from Yutaka Hata & Yoshimi Takai (Addgene plasmid # 47940 ; http://n2t.net/addgene:47940 ; RRID:Addgene_47940) -
For your References section:
SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. Takeuchi M, Hata Y, Hirao K, Toyoda A, Irie M, Takai Y. J Biol Chem. 1997 May 2;272(18):11943-51. 10.1074/jbc.272.18.11943 PubMed 9115257