164,388 results

1. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV9)

   Viral Prep

   #162380-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Tags

   None

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pLVX-IRES-Hygro_GCaMP6s

   Plasmid

   #164591

   Purpose

   Stable integration and expression of GCaMP6s in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-EGFR-G719S

   Plasmid

   #116256

   Purpose

   Lentiviral expression of EGFR G719S

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G719S

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE332

   Plasmid

   #153241

   Purpose

   M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMYT017

   Plasmid

   #180670

   Purpose

   Encodes LacI as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

   Depositor

   Tom Ellis

   Article

   Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

   Insert

   LacI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTKRED

   Plasmid

   #41062

   Depositor

   Edward Cox, Thomas Kuhlman

   Article

   Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6P1-Vinculin tail

   Plasmid

   #107154

   Purpose

   bacterial expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUltra-5HTP

   Plasmid

   #178042

   Purpose

   Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

   Depositor

   Han Xiao

   Article

   Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

   Inserts

   Modified ScTrpRS for 5HTP incoporation
   suppressor tRNA for 5HTP incorporation
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T107C, P254T, C255A

   Promoter

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 4xMS2-hTR

   Plasmid

   #154940

   Purpose

   4xMS2 stem-loop-hTR-500 bp genomic sequence

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   4xMS2 stem-loop-hTR-500 bp genomic sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPSeq-BFP

    Plasmid

    #196718

    Purpose

    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAKgfplux1

    Plasmid

    #14083

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Bacterial luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

    Plasmid

    #74932

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OXTR-Tango

    Plasmid

    #66467

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    OXTR (OXTR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. EGFP-CLC

    Plasmid

    #193559

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

    Plasmid

    #134859

    Purpose

    Expresses EGFP-tagged ERGIC marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    ERGIC53 (Lman1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-hygro CDK4 R24C

    Plasmid

    #11254

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    R24C

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGE333

    Plasmid

    #153242

    Purpose

    M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207860

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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