168,579 results

1. pDONR221-SLC25A3_STOP

   Plasmid

   #161206

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A3 (SLC25A3 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. eGFP-ova-C1

   Plasmid

   #163524

   Purpose

   Expresses both eGFP and chicken ovalbumin in mammalian cells

   Depositor

   Thomas Weber

   Insert

   enhanced-GFP-chicken ovalbumin tandem
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-hChR2(H134R)-EYFP (AAV PHP.eB)

   Viral Prep

   #26973-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   Syn

   Tags

   EYFP

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

4. pHAGE-ERBB2

   Plasmid

   #116734

   Purpose

   Lentiviral expression of ERBB2

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB80-hsKIF5B(1-560)Rigor-L-mVenus

   Plasmid

   #193720

   Purpose

   Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Human, Synthetic)
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJG/BETA MHC promoter

   Plasmid

   #53963

   Purpose

   transgene expression driven by BETA MHC promoter

   Depositor

   Jeffrey Robbins

   Article

   Rindt et al J Biol Chem. 1993 Mar 5;268(7):5332-8.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   beta MHC

   Available Since

   June 30, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Nuclear cpGFP

   Plasmid

   #215707

   Purpose

   Expresses cpGFP in the nucleus

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and NLS

   Expression

   Mammalian

   Mutation

   circularly permuted GFP

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0630

   Plasmid

   #142668

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR1I2 (NR1I2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) eGFP PPP1R8 G4

    Plasmid

    #129021

    Purpose

    Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+) eGFP EML2 G4

    Plasmid

    #129023

    Purpose

    Expresses an eGFP mRNA with the EML2 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-hPAX7 (#348)

    Plasmid

    #78337

    Purpose

    Overexpression of human PAX7 ORF with a N-terminal FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    PAX7 (PAX7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pGCGFP-puro

    Plasmid

    #31266

    Depositor

    Andrew Pierce

    Insert

    GCGFP
    

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167849

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMH-SFB-DYRK1A

    Plasmid

    #101770

    Purpose

    Mammalian expression of DYRK1A fusion protein

    Depositor

    Michael Huen

    Article

    Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

    Insert

    DYRK1A (DYRK1A Human)
    

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-GFP(+30)

    Plasmid

    #199164

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+30)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-TBK1-C-FLAG-HA

    Plasmid

    #131792

    Purpose

    Lentiviral vector expressing WT TBK1 containing STOP codon

    Depositor

    Alicia Pickrell

    Article

    Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

    Insert

    TBK1 WT (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    STOP codon

    Expression

    Mammalian

    Mutation

    WT full length

    Promoter

    CMV

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRha-ABE8e-SpCas9-NG

    Plasmid

    #201190

    Purpose

    Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

    Depositor

    Jonathan Yen

    Article

    Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

    Insert

    ABE8e-SpCas9-NG
    

    Tags

    8xHIS and BP-NLS

    Expression

    Bacterial

    Promoter

    pRhaBAD

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330S-4

    Plasmid

    #58780

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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