1432 pcDNA3 flag MAGI1c
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10714||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)6000
Entrez GeneMagi1 (a.k.a. AIP3, BAP1, Baiap1, Gukmi1, MAGI1c, Magi-1, TNRC19, WWP3, mKIAA4129)
/ Fusion Protein
- flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
See author's map for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:1432 pcDNA3 flag MAGI1c was a gift from William Sellers (Addgene plasmid # 10714 ; http://n2t.net/addgene:10714 ; RRID:Addgene_10714)
For your References section:Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex. Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers WR. J Biol Chem. 2001 Dec 28. 276(52):48627-30. 10.1074/jbc.C100556200 PubMed 11707428