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mTLR2
(Plasmid #13083)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13083 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TLR2
  • Alt name
    toll-like receptor 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • GenBank ID
  • Entrez Gene
    Tlr2 (a.k.a. Ly10, Ly105)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Please note that Addgene's sequencing results indicate that GenBank ID AAH14693.1 is better match for the mouse TLR2 insert sequence in this plasmid. When compared to GenBank ID NM_011905 there is an apparent mutation (M82I) which may represent a valid polymorphism.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTLR2 was a gift from Ruslan Medzhitov (Addgene plasmid # 13083 ; http://n2t.net/addgene:13083 ; RRID:Addgene_13083)