|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13083||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nametoll-like receptor 2
SpeciesM. musculus (mouse)
Insert Size (bp)2300
Entrez GeneTlr2 (a.k.a. Ly10, Ly105)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Please note that Addgene's sequencing results indicate that GenBank ID AAH14693.1 is better match for the mouse TLR2 insert sequence in this plasmid. When compared to GenBank ID NM_011905 there is an apparent mutation (M82I) which may represent a valid polymorphism.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mTLR2 was a gift from Ruslan Medzhitov (Addgene plasmid # 13083 ; http://n2t.net/addgene:13083 ; RRID:Addgene_13083)