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pSS02:cyt-Peredox-mCherry

Plasmid

#161743

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry

Expression

Plant

Promoter

Ubiquitin 10

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBbS15c-RFP

Plasmid

#126198

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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CbS-Flag-TRRAP

Plasmid

#32103

Depositor

Michael Cole, Yardena Samuels

Insert

Transformation/transcription domain-associated protein (TRRAP Human)

Tags

Flag 1x

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRL-TK 4x wt

Plasmid

#11313

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pGW1-Myc-Sarm1

Plasmid

#50707

Purpose

expression of Sarm1 in mammalian cells

Depositor

Yi-Ping Hsueh

Article

Chen et al J Cell Biol. 2011 May 16;193(4):769-84.

Insert

Sarm1 (Sarm1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

Plasmid

#186980

Purpose

mammalian expression of Cluc-Gluc reporter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

Cluc, Gluc

Use

Luciferase

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-ActA-IRES-puromycin-pLVx-EF1a

Plasmid

#177433

Purpose

To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.

Depositor

David Andrews

Insert

ActA tail anchor (actA Listeria monocytogenes)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_pGK_mCherry

Plasmid

#79124

Purpose

Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5

Plasmid

#65244

Purpose

Expression of HA tagged ERK5 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 (MAPK7 Human)

Tags

HA

Expression

Mammalian

Mutation

A409T

Promoter

CMV

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

Plasmid

#167826

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSN001 pME minprom_mCerulean

Plasmid

#195959

Purpose

Gateway pME fluorophore

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter mCerulean

Use

Other

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pmVen-TopBP1

Plasmid

#211413

Purpose

mammalian expression of human TopBP1 N-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

TopBP1 (TOPBP1 Human)

Tags

mVenus

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pNarsenic

Plasmid

#247320

Purpose

Biosensor module for arsenic in E. coli with naringenin-inducible arsR expression

Depositor

Eveline Peeters

Article

Crabbe et al Synth Biol (Oxf). 2026 Jan 10;11(1):ysag

Inserts

fdeR
mkate2
Arsenical resistance operon repressor

Use

Synthetic Biology

Promoter

P_arsOC2, P_fdeA, and P_fdeR

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.mCherry

Plasmid

#248069

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mCherry

Use

AAV

Mutation

S29W.A95K

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.mCherry

Plasmid

#248073

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mCherry

Use

AAV

Mutation

A95A.A119L

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).cpSFGFP.tdTomato

Plasmid

#248076

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9 (D12)

Plasmid

#239488

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available Since

Feb. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_SRF-NLS-VP64 (3'UTR)_RLEloop

Plasmid

#240258

Purpose

Attenuated PROTEUS vector with SRF-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

SRF

Tags

NLS-VP64

Expression

Mammalian

Mutation

SRF[aa1-265]; VP64 = 4*VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_p53-ZF43

Plasmid

#240263

Purpose

Constitutive expression (CMV promoter) of a fusion of the p53 DNA-binding domain and ZF43. Used as part of the circuit for Nb139-VP64 transgene activity.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

p53-ZF43

Expression

Mammalian

Mutation

p53[aa93-393]

Promoter

CMV

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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