Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

179,241 results

Showing: 7701 - 7720 of 179241 results

  2. pBMN-Z-IN

    Plasmid
    #1735
    Purpose
    This retroviral vector expresses lacZ and has an IRES-NEO downstream
    Depositor
    Garry Nolan
    Insert
    LacZ-IRES-Neo
    Use
    Retroviral
    Available since
    Feb. 14, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    1735-plasmid-map-sequence-id-336

    Enlarge

  4. 401 pSG5L

    Plasmid
    #10737
    Depositor
    William Sellers
    Type
    Empty backbone
    Expression
    Mammalian
    Available since
    Sept. 27, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  6. AAV-hSyn-Soma-jGCaMP8m (AAV1)

    Viral prep
    #169257-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Marianne Fyhn
    Publication
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Available since
    July 21, 2023
    Availability
    Academic Institutions and Nonprofits only

  7. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    193967-plasmid-map-sequence-id-381640

    Enlarge

  8. pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE (AAV1)

    Viral prep
    #179459-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE (#179459). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE plasmid DNA. EF1a-driven, Cre-dependent soma and AIS-localized expression of voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Francois St-Pierre
    Publication
    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00
    Promoter
    EF1a
    Tags
    cEGFP (Cre-dependent)
    Available since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only

  9. nuclear NAD+ biosensor

    Plasmid
    #186789
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus
    Depositor
    Michael Cohen
    Publication
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    nuclear NAD+ biosensor
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    186789-plasmid-map-sequence-id-368062

    Enlarge

  10. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

    Enlarge

  13. pAAV-hSyn-DIO-rM3D(Gs)-mCherry (AAV8)

    Viral prep
    #50458-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-rM3D(Gs)-mCherry (#50458). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-rM3D(Gs)-mCherry plasmid DNA. hSyn-driven, Cre-dependent rM3D(Gs) receptor with an mCherry reporter for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid
    #177672
    Purpose
    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells
    Depositor
    Karl Deisseroth
    Has service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    nEF
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    177672-plasmid-map-sequence-id-348533

    Enlarge

  17. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV Retrograde)

    Viral prep
    #162379-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)

    Viral prep
    #137128-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 7701 - 7720 of 179241 results