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Base editing with a Cpf1-cytidine deaminase fusion.
Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, Huang X, Yang L, Chen J
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
107678pCMV-dCpf1-BE0Expresses dLbCpf1-BE0 in mammalian cells
107680pST1374-Lb-Cpf1-NLSExpresses LbCpf1 in mammalian cells
107682pLb-Cpf1-pGL3-U6-sgRNAExpresses LbCpf1 crRNA in mammalian cells
107683pAs-Cpf1-pGL3-U6-sgRNAExpresses AsCpf1 crRNA in mammalian cells
107684pCMV-dLbCpf1-BE0ΔiNLSExpresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells
107685pCMV-dCpf1-BEExpresses dLbCpf1-BE in mammalian cells
107686pCMV-dCpf1-BE-YEExpresses dLbCpf1-BE-YE in mammalian cells
107687pCMV-dCpf1-BE-YEEExpresses dLbCpf1-BE-YEE in mammalian cells
107688pCMV-dCpf1-eBEExpresses dLbCpf1-eBE in mammalian cells
107689pCMV-dCpf1-eBE-YEExpresses dLbCpf1-eBE-YE in mammalian cells

Antibodies from Article