179,756 results
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Plasmid#153285PurposeExpress the adenosine sensor GRAB_Ado1.0 in neuronsDepositorHas serviceAAV1InsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterhSynAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA4mt
Plasmid#140463PurposeRed fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertR-CEPIA4mt
TagsMycExpressionMammalianAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-All-in-one-hCascade
Plasmid#134919PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
ExpressionMammalianPromoterCAGAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT3-EF1A-MYC-IRES-lucOS
Plasmid#129776PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman MYC (MYC Human)
TagsOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…ExpressionMammalianPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MLRV
Plasmid#118069PurposeMulti-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reportersDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTB:5xNF-κβ:RedF:bGHpA
TB:4xTGF-β:FLuc:bGHpA
TB:5xE-box:Renilla:bGHpA
TB:2xp53:NLuc:bGHpA
TB:6xAP-1:GrRenilla:bGHpA
CMV:ELuc:bGHpA
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoter2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-Puro-TDP-43-WT
Plasmid#133753PurposeWild type human TDP-43 in pLVX-Puro lentiviral vectorDepositorAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLCKO2::TKOv3
Pooled library#125517PurposeHuman CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backboneDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRIP-SFFV-EGFP-FLAG-cGAS
Plasmid#127661PurposeOver-express EGFP-FLAG-cGASDepositorAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CDH5-AbR-GFP
Plasmid#122970PurposeLenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.DepositorInsertsCDH5p
CopGFP
Zeocin
UseLentiviralExpressionMammalianMutationNAAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5
Plasmid#124362PurposeOverexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)DepositorInsertTornado-F30-Broccoli-aptNFkB#5
ExpressionMammalianPromoterU6+27Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SMARCA2-GFP
Plasmid#65390PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMARCA2 (SMARCA2 Human)
TagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GATA3-pCDNA3.1
Plasmid#115271PurposeExpresses human GATA3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGATA3 (GATA3 Human)
ExpressionMammalianPromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZS165 CRISPEY HH-HDV NotI
Plasmid#114451PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsExpressionYeastPromoterScGAL7pAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
Plasmid#115267PurposeExpresses LbuCas13a from a SUMO-tagged bacterial expression constructDepositorInsertLbuCas13a
TagsSUMOExpressionBacterialAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-dLight1.2
Plasmid#111068PurposeTo generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoterDepositorHas serviceAAV1 and AAV5InsertdLight1.2
UseAAVTagsFlag tagAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediatePromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pScaf
Plasmid#111401PurposePhagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18DepositorTypeEmpty backboneExpressionBacterialAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VEGFR2-mCh
Plasmid#108854PurposeEncodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKDR (KDR Human)
TagsLabeled with mCherry on the C-Terminus via a 3 am…ExpressionMammalianAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX601 miniCMV-SaCas9-SpA-sgRNA scaffold
Plasmid#107055PurposeBackbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)DepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-7 asyn A53T
Plasmid#105727PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by