174,897 results

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CL20_mEGFP_TCF3_HLF

Plasmid

#205935

Purpose

Express mEGFP-tagged fusion protein, TCF3_HLF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TCF3_HLF (TCF3, HLF Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-FBXL11

Plasmid

#126543

Purpose

Expresses GFP-tagged FBXL11 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

FBXL11 (KDM2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_CLOCK_WT

Plasmid

#82247

Purpose

Gateway Donor vector containing CLOCK , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CLOCK (CLOCK Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DHFR.cHSF1

Plasmid

#134738

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available Since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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mVenus N1

Plasmid

#27793

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Has Service

Cloning Grade DNA

Insert

mVenus N1

Expression

Mammalian

Available Since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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APEX2 - Tubulin in pEGFP

Plasmid

#66171

Purpose

APEX2 fused to the N-terminus of alpha tubulin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2 - alpha tubulin

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1_Cas9-hGem_gRNA2

Plasmid

#217659

Purpose

expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA2 targeting the human AAVS1 safe harbour locus

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR

Plasmid

#64927

Purpose

FRET-based biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR (EIF4EBP1 Synthetic, Human)

Tags

Cerulean and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRK1043

Plasmid

#8835

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150

Plasmid

#160992

Purpose

Express fulllength of mouse p150 protein

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPlatTET-gRNA2

Plasmid

#82559

Purpose

All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD

Expression

Mammalian

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSens-4OmeNB

Plasmid

#216231

Purpose

Expresses GFP in response to 4'-O-Methylnorbelladine

Depositor

Simon d'Oelsnitz, Andrew Ellington

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Inserts

RamR-4NB2
sfGFP

Use

Synthetic Biology

Mutation

K64T, L67M, C135D, S138G

Promoter

J23103 and Pramr

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217345

Purpose

crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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FMR1-E17C-3xFLAG

Plasmid

#157784

Purpose

donor plasmid for C-terminal FLAG tag fusion of human FMR1

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1-FLAG (FMR1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

Plasmid

#105556

Purpose

AAV expression of EGFP from tMCK promoter

Depositor

James M. Wilson

Has Service

AAV9

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

tMCK

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA-UGI

Plasmid

#221220

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-CIP2A

Plasmid

#222623

Purpose

mammalian expression vector of Flag-tagged CIP2A

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF0988

Plasmid

#143455

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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