PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||126049||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 5227
Vector typePCR-based C-terminal tagging
Selectable markersKanMX6 (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (unknown if destroyed)
- 3′ cloning site PacI (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFA6a-TurboID-3MYC-kanMX6 (pFB1420) was a gift from François Bachand (Addgene plasmid # 126049 ; http://n2t.net/addgene:126049 ; RRID:Addgene_126049)
For your References section:Proximity-dependent biotinylation by TurboID to identify protein-protein interaction networks in yeast. Larochelle M, Bergeron D, Arcand B, Bachand F. J Cell Sci. 2019 May 7. pii: jcs.232249. doi: 10.1242/jcs.232249. 10.1242/jcs.232249 PubMed 31064814