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mcherry-DN KASH
(Plasmid #125553)


Item Catalog # Description Quantity Price (USD)
Plasmid 125553 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pmcherry N1
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 4900
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Truncated form: only the KASH domain of nesprin-1 is expressed to create a dominant negative
  • GenBank ID
  • Entrez Gene
    SYNE1 (a.k.a. 8B, AMC3, AMCM, ARCA1, C6orf98, CPG2, EDMD4, KASH1, MYNE1, Nesp1, SCAR8, dJ45H2.2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mcherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The KASH-1 sequence was synthetically produced by GeneArt.
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mcherry-DN KASH was a gift from Daniel Conway (Addgene plasmid # 125553 ; ; RRID:Addgene_125553)
  • For your References section:

    Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells. Mayer CR, Arsenovic PT, Bathula K, Denis KB, Conway DE. Cell Mol Bioeng. 2019 Jul 11;12(4):289-300. doi: 10.1007/s12195-019-00579-y. eCollection 2019 Aug. 10.1007/s12195-019-00579-y PubMed 31719915