167,468 results

1. TRE-mCherry-p2A-eNpHR3.0-TS

   Plasmid

   #158703

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   mCherry-p2A-eNpHR3.0-TS
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTet-IRES-EGFP

   Plasmid

   #64238

   Purpose

   Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

   Depositor

   Maria Lung

   Article

   Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   pTet

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p7SL-neo-TET

   Plasmid

   #51285

   Purpose

   expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   7SL RNA (RN7SL2 Human)
   

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Promoter

   7SL upstream pollII enhancer

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEB2-E2-Crimson

   Plasmid

   #104010

   Purpose

   Plasmid encoding E2-Crimson

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   E2-Crimson
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-VEGFR2-V5/HIS

   Plasmid

   #205617

   Purpose

   Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR2 receptor tyrosine kinase (KDR Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS-mCherry

   Plasmid

   #41583

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. qTAG-C-3xFLAG-Blast

   Plasmid

   #207717

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pLenti6.2_miRFP670

   Plasmid

   #113726

   Purpose

   Expresses the fluorescent protein miRFP670 in a lentiviral backbone

   Depositor

   Vanessa LaPointe

   Insert

   miRFP670
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

   Plasmid

   #198753

   Purpose

   Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

   Depositor

   Jacco van Rheenen

   Article

   Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

   Insert

   EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMCSG53-DsbC

    Plasmid

    #186623

    Purpose

    E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

    Depositor

    Alexei Savchenko

    Article

    Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

    Type

    Empty backbone

    Tags

    DsbC-6His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE-ESR1-Y537S

    Plasmid

    #116374

    Purpose

    Lentiviral expression of ESR1 Y537S

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Mutation

    Y537S

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 puro Nodamura B2

    Plasmid

    #17228

    Depositor

    Christopher Sullivan

    Article

    Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

    Insert

    Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)
    

    Expression

    Mammalian

    Mutation

    2 silent point mutations

    Available Since

    June 22, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pENTR-ERKKTRClover

    Plasmid

    #59138

    Purpose

    ORF encoding ERK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
    

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFUGW SpCas9

    Plasmid

    #131506

    Purpose

    lentiviral expression of SpCas9 under hUBC promotor

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    SpCas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUBC

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICH-nuc-roGFP2-ORP1

    Plasmid

    #191758

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCX-DI-2A

    Plasmid

    #74671

    Purpose

    Mammalian expression of DI-2A (hCD73/F2A/hCD39)

    Depositor

    Roberto Giovannoni

    Article

    De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

    Inserts

    CD73 (NT5E Human)
    CD39 (ENTPD1 Human)
    

    Tags

    F2A

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmTurquoise2-Drp1

    Plasmid

    #110946

    Purpose

    Expresses dynamin-like protein 1 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Dynamin-1-like protein (DNM1L Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mNeonGreen-JIP4

    Plasmid

    #164621

    Purpose

    Expresses JIP4 in mammalian cells with a mNeonGreen tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    JIP4 (SPAG9 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1973

    Plasmid

    #142519

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX3 (TBX3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-hCDC6p-Venus-puro

    Plasmid

    #212666

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Promoter

    hCDC6p

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge