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Showing: 7821 - 7840 of 160228 results

  5. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  6. FUW-hR3-EGFP

    Plasmid
    #119231
    Purpose
    Plasmid encodes full-length human VEGFR-3 gene fused in-frame with EGFP cloned in lentiviral vector.
    Depositor
    Kari Alitalo
    Insert
    VEGFR3-EGFP (FLT4 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    P hUbC
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    119231-plasmid-map-sequence-id-231731

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  7. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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Showing: 7821 - 7840 of 160228 results