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177,963 results

Showing: 7821 - 7840 of 177963 results

  1. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

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  2. pQUAST-attB

    Plasmid
    #104880
    Purpose
    For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system
    Depositor
    Christopher Potter
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    March 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    104880-plasmid-map-sequence-id-204757

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  10. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

    Viral Prep
    #92379-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .
    Depositor
    John Doench, David Root
    Article
    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:
    Available Since
    Aug. 3, 2017
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid
    #84446
    Purpose
    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has Service
    AAV1, AAV5, and AAV8
    Insert
    Jaws-KGC-tdTomato-ER2
    Use
    AAV
    Tags
    ER2, KGC, and tdTomato
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available Since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    84446-plasmid-map-sequence-id-317050

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  17. AICSDP-7:SEC61B-mEGFP

    Plasmid
    #87426
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87426-plasmid-map-sequence-id-173967

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  18. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

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Showing: 7821 - 7840 of 177963 results