166,818 results

1. pcDNA3.1(+) EGFP-Rap1A(WT)

   Plasmid

   #224276

   Purpose

   Cell transfection and expression of Rap1A

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rap1A (RAP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221076

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221077

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221079

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. DVC_pBR322_AF

   Plasmid

   #217598

   Purpose

   Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. DVC_p15A_AF

   Plasmid

   #217588

   Purpose

   Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Bcl-2-ΔBH4

   Plasmid

   #220300

   Purpose

   mammalian expression of Bcl-2-ΔBH4 tagged with GFP

   Depositor

   Xinlai Cheng

   Article

   Wang et al Cell Reports Physical Science, Volume 5,

   Insert

   Bcl2 (BCL2 Human)
   

   Use

   Tags

   Emerald GFP

   Expression

   Mammalian

   Mutation

   BH4 deleted

   Promoter

   CMV

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYSD061

   Plasmid

   #212915

   Purpose

   Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SUC2 (SUC2 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDO068

   Plasmid

   #198788

   Purpose

   Drives specific expression in the BAG neurons

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   Pgcy-33-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV2ss-U6-sgHTT1-7sk-sgCas9

    Plasmid

    #190900

    Purpose

    AAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAs

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA (HTT spCas9, Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and 7sk

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-SIGLEC8-COMP5AP-AviTag-9xHis

    Plasmid

    #157356

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIGLEC8 (SIGLEC8 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-SIGLEC15-COMP5AP-AviTag-9xHis

    Plasmid

    #157262

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIGLEC15 (SIGLEC15 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV Myc-MBP

    Plasmid

    #175103

    Purpose

    Lentiviral expression of Myc-tagged MBP

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    MBP
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYCTK027

    Plasmid

    #176731

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Saccharomyces cerevisiae (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Sc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYCTK002

    Plasmid

    #176706

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS1 of S. cerevisiae (response part)

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    P-FUS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

    Plasmid

    #171100

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Neurog2 S. pyogenes, Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF3532

    Plasmid

    #145008

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LIN28B (LIN28B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-Cre (AAV11)

    Viral Prep

    #55636-AAV11

    Purpose

    Ready-to-use AAV11 particles produced from pAAV-EF1a-Cre (#55636). In addition to the viral particles, you will also receive purified pAAV-EF1a-Cre plasmid DNA. EF1a-driven expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    EF1a

    Tags

    None

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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