252,515 results

1. pLenti-sgRNA(lib)-puro

   Plasmid

   #119976

   Purpose

   Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Wensheng Wei

   Article

   Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGFP-Cytochrome C

   Plasmid

   #41181

   Depositor

   Douglas Green

   Article

   Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

   Insert

   Cytochrome C (CYCS Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGFP-Cytochrome C

   Plasmid

   #41181

   Depositor

   Douglas Green

   Article

   Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

   Insert

   Cytochrome C (CYCS Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSBbi_RP_3XNLS-MicroID2-FLAG

   Plasmid

   #188523

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   3XNLS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-Puro-TDP-43-K263E

   Plasmid

   #133758

   Purpose

   Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed lysine 263 to glutamate.

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Puro-TDP-43-K263E

   Plasmid

   #133758

   Purpose

   Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed lysine 263 to glutamate.

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLminP_Luc2P_RE59

   Plasmid

   #90404

   Purpose

   STAT3 - Sis-inducible element (SIE) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   STAT3

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLminP_Luc2P_RE59

   Plasmid

   #90404

   Purpose

   STAT3 - Sis-inducible element (SIE) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   STAT3

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO5.sgRNA.EFS.tRFP657

   Plasmid

   #57824

   Purpose

   Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP657
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO5.sgRNA.EFS.tRFP657

    Plasmid

    #57824

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AbVec2.0-mIghg1

    Plasmid

    #127158

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg1 (Ighg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AbVec2.0-mIghg1

    Plasmid

    #127158

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg1 (Ighg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DJ-1 pET15b

    Plasmid

    #60678

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. DJ-1 pET15b

    Plasmid

    #60678

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

15. VinTS-tCRMod-GGSGGS9

    Plasmid

    #111765

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VinTS-tCRMod-GGSGGS9

    Plasmid

    #111765

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB402-omega

    Plasmid

    #74797

    Purpose

    Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2xCaMV35S enhancer and omega

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWB402-omega

    Plasmid

    #74797

    Purpose

    Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2xCaMV35S enhancer and omega

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-MXT GIRK2

    Plasmid

    #20675

    Depositor

    Henry Lester

    Article

    Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

    Insert

    Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
    

    Use

    Xenopus expression

    Tags

    3' UTR Xenopus B- globin and 5' UTR Xen…

    Expression

    Mutation

    A369V

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-MXT GIRK2

    Plasmid

    #20675

    Depositor

    Henry Lester

    Article

    Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

    Insert

    Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
    

    Use

    Xenopus expression

    Tags

    3' UTR Xenopus B- globin and 5' UTR Xen…

    Expression

    Mutation

    A369V

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge