Skip to main content
Holiday Schedule: Addgene will be closed November 26th & 27th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about your shipment please contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H
Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50363AAV phSyn1(S)-DreO-bGHpACan be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter
Loading...
50364AAV phSyn1(S)-FlpE-bGHpACan be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter
Loading...
51507AAV pmSyn1-EBFP-CreCan be used to generate AAV virus that will express Cre in neurons from the synapsin promoter
Loading...
51669AAV phSyn1(S)-FlpO-bGHpACan be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.
Loading...
61570Pvalb-2A-Cre targeting vectorTarget the Cre recombinase gene to the stop codon of the mouse Pvalb gene
Loading...
61571Pvalb-2A-Dre Flp-in replacement vectorRecombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon
Loading...
61572Pvalb-2A-Flpo Flp-in replacement vectorRecombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon
Loading...
61573Rasgrf2-2A-dCre targeting vectorTarget a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene
Loading...
61574Slc17a7-IRES2-Cre targeting vectorTarget the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene
Loading...
61575Snap25-LSL-2A-GFP targeting vectorTarget a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene
Loading...
61576Ai62(TITL-tdT) Flp-in replacement vectorRecombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
Loading...
61577Ai65(RCFL-tdT) targeting vectorTarget a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus
Loading...
61578Ai66(RCRL-tdT) targeting vectorTarget a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus
Loading...
61579Ai95(RCL-GCaMP6f) targeting vectorTarget a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus
Loading...
61580TIGRE docking site targeting vectorCreate a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange
Loading...
65429AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpACan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases
Loading...
65449AAV pTRE-FLEX-EGFP-WPRE-bGHpACan be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner
Loading...
65453AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpACan be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases
Loading...
65454AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpACan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases
Loading...
65455AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpACan be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases
Loading...
65458AAV pCAG-FLEX2-tTA2-WPRE-bGHpACan be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner
Loading...